The "Programmer" selection in the "Tools" menu applies to burning bootloaders. Sketch uploads generally use what is selected in "Serial Port".
What are you trying to accomplish here? If you have already burned a bootloader, you don't need the USBTinyISP to upload sketches. You can do so directly via a USB/COM port. If you want to burn a sketch to a bare chip, then you don't need to burn the bootloader.
For information on burning sketches without a bootloader, see: http://www.arduino.cc/en/Hacking/Programmer